Oral l-citrulline supplementation improves erectile function and penile structure in

mercredi 1 janvier 2014

Related Articles Oral l-citrulline supplementation improves erectile function and penile structure in castrated rats.



Int J Urol. 2013 Dec 23;



Authors: Hotta Y, Shiota A, Kataoka T, Motonari M, Maeda Y, Morita M, Kimura K



Abstract

OBJECTIVES: To investigate the efficacy of oral l-citrulline for erectile dysfunction and penile structure disruption in a rat model.

METHODS: Male Wistar-ST rats aged 15 weeks were randomly divided into three groups as follows: sham-operated rats (control group), surgically castrated rats (castrated group) and surgically castrated rats subsequently treated with 2% l-citrulline water (castrated + l-citrulline). At 4 weeks postoperative, erectile function was assessed based on intracavernous pressure changes, followed by electrostimulation of cavernous nerves and calculation of maximum intracavernous pressure/mean arterial pressure. Penile structure was evaluated by Masson's trichrome staining and the smooth muscle-to-collagen ratio was calculated. The serum bioavailable testosterone, l-arginine, l-citrulline, N(G) ,N(G) -dimethylarginine and nitrogen oxide levels were evaluated.

RESULTS: The bioavailable testosterone concentrations were decreased in the castrated and castrated + l-citrulline groups compared with the control group at 4 weeks after surgery. The intracavernous pressure-to-mean arterial pressure and smooth muscle-to-collagen ratios were significantly decreased in the castrated group compared with the control group, but significantly increased in the castrated + l-citrulline group compared with the castrated group. The serum l-citrulline, l-arginine and N(G) ,N(G) -dimethylarginine levels, and the l-arginine-to-N(G) ,N(G) -dimethylarginine ratios were significantly increased in the castrated + l-citrulline group compared with the castrated group. The serum nitrogen oxide levels were increased in the castrated + l-citrulline group compared with the castrated group.

CONCLUSIONS: Oral l-citrulline can improve the erectile response to electric stimulation of cavernous nerve and penile structure in castrated rats.





PMID: 24372616 [PubMed - as supplied by publisher]







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